In my first pack, I applied the Fish Paper to all sides/bottom of every cell, then put the cells into the cases.
On the next three DIY packs, I just applied the Fish Paper between adjoining cells, one layer between each cell. This seems good enough to me, and as Gotbeans said, totally different for a mobile battery set, where you know there will be vibration/dynamic forces on the cells leading to abrasion concerns. When I look at automotive cells, they don't seem to use FR4 between cells, they use adhesives, and mechanical clamping systems to keep the cells firmly secured.